Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Invitrogen molecular biology grade, TE, pH 8.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use.

Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.

Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.

Molecular biology grade, Invitrogen 20X SSPE solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.

Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.

Invitrogen™ RNA Rapid Extraction Solution is to release RNA from the paper prior to RNA isolation. 10 mL is provided.

Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.

UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.

For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency.

Ambion Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ±0.2, with included buffer).

Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon.

Molecular biology grade, Invitrogen 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use.

Invitrogen TE buffer is a commonly recommended storage solution for RNA samples. 10 mM Tris-HCl, 1 mM EDTA, pH 7.0 is in nuclease-free, ultrapure water.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Precipitation solution offers major advantages over other RNA precipitation methods in that it does not precipitate DNA, protein, or carbohydrates.

Highest grade available

Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.

Molecular biology grade, Invitrogen SDS, 10% Solution is supplied in one bottle containing 500 mL.

GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.

The same high-quality deoxynucleotide triphosphate solutions provided in the Invitrogen™ RETROscript™ First Strand cDNA Synthesis Kit for RT-PCR.

Invitrogen IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage.

UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.